WebNov 19, 2015 · Interestingly, blue-shifted iRFP670 and iRFP682 have higher quantum yields than their respective red-shifted NIR FP variants. Here we developed a novel NIR FP, named BphP1-FP, from wild-type Rp BphP1. BphP1-FP exhibits the most blue-shifted absorbance and fluorescence emission and the highest quantum yield among the available BphP … WebJun 1, 2024 · The miRFP series, consisting of miRFP670, miRFP703, and miRFP709 NIR FPs, allowed the protein labeling and development of biosensors in the NIR spectral region. miRFPs are of special interest for structural and biochemical studies, since they evolved from the same RpBphP1 template and differ only by several key amino-acid residues that …
Designing brighter near-infrared fluorescent proteins: insights …
WebThe spectral and biochemical properties of the iRFP670, iRFP682, iRFP713 proteins and … WebIn miRFP670, a second C–S covalent bond formation to a cysteine in the PAS domain occurred in 14 min, providing a rigid tetrapyrrole structure with high brightness. Our findings provide insights... huggingface wav2vec2 chinese
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WebiRFP670 Species Rhodopseudomonas palustris Insert Size (bp) 936 Mutation T2A, S45T, … WebDec 26, 2024 · iRFP670, and mIFP. Our results show that both mCardinal and iRFP670 are useful fluorescent ... 2013), it is advantageous to consider complementary FPs whose spectra lie in the far-red and/or near-infrared region (where 650 nm is an approximate boundary between far-red <650, and near-infrared >650; ... WebiRFP670 1 .. 936 = 936 bp 311 amino acids = 34.5 kDa Product: near-infrared fluorescent protein with an emission peak at 670 nm, engineered from a bacterial phytochrome (Shcherbakova and Verkhusha, 2013) derived from Rhodopseudomonas palustris RpBphP6 iRFP670 1 .. 936 = 936 bp 311 amino acids = 34.5 kDa holiday homes gairloch scotland